A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP
Identifieur interne : 000956 ( Main/Exploration ); précédent : 000955; suivant : 000957A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP
Auteurs : Nattamon Siboonnan [Thaïlande] ; Wittawat Wiriyarat [Thaïlande] ; Chompunuch Boonarkart [Thaïlande] ; Warunya Chakritbudsabong [Thaïlande] ; Anan Jongkaewwattana [Thaïlande] ; Pilaipan Puthavathana [Thaïlande] ; Prasert Auewarakul [Thaïlande] ; Ornpreya Suptawiwat [Thaïlande]Source :
- Archives of Virology [ 0304-8608 ] ; 2013-06-01.
Abstract
Abstract: We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells.
Url:
DOI: 10.1007/s00705-012-1595-1
Affiliations:
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of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, 10700, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Chakritbudsabong, Warunya" sort="Chakritbudsabong, Warunya" uniqKey="Chakritbudsabong W" first="Warunya" last="Chakritbudsabong">Warunya Chakritbudsabong</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Preclinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, 73170, Nakhonpathom</wicri:regionArea><wicri:noRegion>Nakhonpathom</wicri:noRegion></affiliation></author><author><name sortKey="Jongkaewwattana, Anan" sort="Jongkaewwattana, Anan" uniqKey="Jongkaewwattana A" first="Anan" last="Jongkaewwattana">Anan Jongkaewwattana</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>National Center of Genetic Engineering and Biotechnology, 12120, Pathumthani</wicri:regionArea><wicri:noRegion>Pathumthani</wicri:noRegion></affiliation></author><author><name sortKey="Puthavathana, Pilaipan" sort="Puthavathana, Pilaipan" uniqKey="Puthavathana P" first="Pilaipan" last="Puthavathana">Pilaipan Puthavathana</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, 10700, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Auewarakul, Prasert" sort="Auewarakul, Prasert" uniqKey="Auewarakul P" first="Prasert" last="Auewarakul">Prasert Auewarakul</name><affiliation wicri:level="1"><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, 10700, 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when="2013-06-01">2013-06-01</date><biblScope unit="volume">158</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1151">1151</biblScope><biblScope unit="page" to="1157">1157</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells.</div></front></TEI><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Siboonnan, Nattamon" sort="Siboonnan, Nattamon" uniqKey="Siboonnan N" first="Nattamon" last="Siboonnan">Nattamon Siboonnan</name></noRegion><name sortKey="Auewarakul, Prasert" sort="Auewarakul, Prasert" uniqKey="Auewarakul P" first="Prasert" last="Auewarakul">Prasert Auewarakul</name><name sortKey="Boonarkart, Chompunuch" sort="Boonarkart, Chompunuch" uniqKey="Boonarkart C" first="Chompunuch" last="Boonarkart">Chompunuch Boonarkart</name><name sortKey="Chakritbudsabong, Warunya" sort="Chakritbudsabong, Warunya" uniqKey="Chakritbudsabong W" first="Warunya" last="Chakritbudsabong">Warunya Chakritbudsabong</name><name sortKey="Jongkaewwattana, Anan" sort="Jongkaewwattana, Anan" uniqKey="Jongkaewwattana A" first="Anan" last="Jongkaewwattana">Anan Jongkaewwattana</name><name sortKey="Puthavathana, Pilaipan" sort="Puthavathana, Pilaipan" uniqKey="Puthavathana P" first="Pilaipan" last="Puthavathana">Pilaipan Puthavathana</name><name sortKey="Suptawiwat, Ornpreya" sort="Suptawiwat, Ornpreya" uniqKey="Suptawiwat O" first="Ornpreya" last="Suptawiwat">Ornpreya Suptawiwat</name><name sortKey="Wiriyarat, Wittawat" sort="Wiriyarat, Wittawat" uniqKey="Wiriyarat W" first="Wittawat" last="Wiriyarat">Wittawat Wiriyarat</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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